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SRX3070964: GSM2735594: AXL KO1 (tumor graft); Mus musculus; RNA-Seq
1 ILLUMINA (Illumina HiSeq 2000) run: 34.8M spots, 3.5G bases, 1.4Gb downloads

Submitted by: NCBI (GEO)
Study: RNA-seq of MMTV-Neu AXL KO tumor grafts in FVB
show Abstracthide Abstract
AXL is activated by its ligand GAS6 and is expressed in triple-negative breast cancer cells. We report that AXL is also detected in HER2+ breast cancer specimens where its expression correlates with poor patients' survival. Using murine models of HER2+ breast cancer, AXL, but not Gas6, was found essential for metastasis. We determined that AXL is required for intravasation, extravasation and growth at the metastatic site. AXL is expressed in HER2+ cancers displaying EMT signatures and contributes to sustain EMT in murine tumors. Interfering with AXL in patient-derived xenograft impaired TGF-ß-induced cell invasion. Lastly, pharmacological inhibition of AXL decreased the metastatic burden of mice developing HER2+ breast cancer. Our data identify AXL as a potential co-therapeutic target during the treatment of HER2+ breast cancers to limit metastasis. Overall design: Differential gene expression profile between tumor grafts of AXL-/- and AXL+/+ cells in FVB mice by RNA sequencing (Illumina HiSEq 2000)
Sample: AXL KO1 (tumor graft)
SAMN07461308 • SRS2415290 • All experiments • All runs
Organism: Mus musculus
Library:
Instrument: Illumina HiSeq 2000
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: PAIRED
Construction protocol: Total RNA was extracted using Trizol reagent and RNA samples were further purified using RNeasy columns (Qiagen). RNA libraries were prepared for sequencing using standard Illumina protocols
Experiment attributes:
GEO Accession: GSM2735594
Links:
Runs: 1 run, 34.8M spots, 3.5G bases, 1.4Gb
Run# of Spots# of BasesSizePublished
SRR590951334,826,0923.5G1.4Gb2018-05-01

ID:
4355239

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